α-Mannosidase Assay Kit
Cat.No. : | Kit-2079 |
Product Overview : | The nonradioactive, colorimetric AMA assay is based on the cleavage of 4- nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity. |
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Size : | 100 tests |
Applications : | For quantitative determination of α-mannosidase enzyme activity using a p-nitrophenol based substrate. |
Storage : | The kit is shipped at room temperature. Store all components at 4°C upon receiving. Shelf life: 6 months after receipt. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMA contributes to the regulation of mitochondrial calcium homeostasis, affecting cellular signaling pathways involved in calcium-dependent processes, such as apoptosis, metabolism, or mitochondrial permeability transition.
The expression or activity of AMA can be regulated in response to physiological cues, such as nutrient availability, energy status, or oxidative stress. The transcriptional or post-transcriptional regulation of AMA involves transcription factors, microRNAs, or epigenetic modifications.
AMA can undergo phosphorylation, acetylation, or ubiquitination, which may affect its stability, subcellular localization, or interactions with other mitochondrial proteins.
AMA interacts with various mitochondrial proteins, such as components of the mitochondrial respiratory chain, mitochondrial transporters, or proteins involved in mitochondrial quality control and dynamics.
The AMA protein localizes to the mitochondria, specifically to the inner mitochondrial membrane or the intermembrane space. Its localization may be dynamic and responsive to cellular signals or stress conditions.
AMA regulates cellular metabolism, oxidative stress response, and cell survival through its influence on mitochondrial function, energy production, reactive oxygen species (ROS) generation, or apoptotic signaling pathways.
AMA plays a role in mitochondrial biogenesis by regulating mitochondrial DNA replication, transcription, or protein synthesis. It also contributes to the maintenance of mitochondrial fusion-fission dynamics.
Dysregulation or mutations in AMA have been associated with mitochondrial disorders, neurodegenerative diseases, or metabolic disorders. Its altered expression or activity may serve as a diagnostic or prognostic marker in these conditions.
Pharmacological modulation of AMA could potentially impact mitochondrial function and may have therapeutic implications for mitochondrial diseases, neurodegenerative disorders, or metabolic disorders.
AMA is involved in the maintenance of mitochondrial DNA integrity by participating in mtDNA replication, repair, and protection against damage caused by oxidative stress or genotoxic agents.
Customer Reviews (3)
Write a reviewWide linear range for accurate protein quantification.
Consistent results in protein-protein interaction mapping.
Minimal batch-to-batch variation in reagent performance.
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