1,5-Anhydroglucitol Cell-Based Uptake Assay Kit

• Cat.No.: Kit-2115
• Product Overview: 1,5-Anhydroglucitol Uptake Assay Kit utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells.

Specification

• Size: 50 assays
• Applications: Measurement of 1,5-Anhydroglucitol uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.Dual-staining of 1,5-AG transporters and 1,5-AG uptake Analysis of 1,5-AG metabolism and cell signaling in various cell typesScreening of anti-diabetic compounds
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Analysis Buffer: Ready to use. Keep on ice while in use. Store at -20 °C.AGTracker Reagent (100X): Aliquot and store at -20°C. For consistent results, avoid repeated freeze/thaw.Phloretin (100X): Bring to room temperature. Ready to use. Store at -20°C.