15-PGDH Inhibitor Screening Kit

• Cat.No.: Kit-2098
• Product Overview: In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing NAD. NADH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=535/587 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence. The 15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.

Specification

• Size: 100 assays
• Applications: Screening/studying/characterizing 15-PGDH inhibitors.
• Storage: Store kit at -20°C, protected from light. Briefly spin small vials prior to opening. Read entire protocol before performing the assay.15-PGDH Assay Buffer: Bring to room temperature before use. Store at -20°C. Use within two months.PicoProbe: Before use, thaw at room temperature. Store at -20°C. Use within two months.15-PGDH Developer: Reconstitute with 220 µl of Assay Buffer. Avoid repeated freeze/thaw. Use within two months. Keep on ice while in use.15-PGDH Substrate and 15-PGDH Inhibitor Control: Before use, thaw at room temperature. Store at -20°C. Use within two months.15-PGDH Enzyme: Ready to use. Keep on ice while in use. Store at -20°C. Use within two months.