Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > 2-Deoxyglucose (2DG) Uptake Measurement Kit II

2-Deoxyglucose (2DG) Uptake Measurement Kit II

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Cat.No.:
Kit-0002
Product Overview:
Direct measurement of 2DG6P without the use of radioisotope.
Description:
Measurement of 2-deoxyglucose (2DG) uptake in tissues and cells is a reliable approach with which to estimate glucose uptake and thereby to explore the regulation of glucose metabolism and mechanism of insulin resistance. Radioisotope-labeled 2DG is usually used for the measurement of 2DG uptake both in vivo and in vitro. However the radioisotope (RI) method is required a specialized facility for RI in strict limitation and cannot be handled in ordinal laboratories. Furthermore, radioactive 2DG administered into cultured cells remains in the extracellular space, and therefore the results must be corrected by separating the extracellular 2DG and intracellular 2DG/2DG-6-phosphate (2DG6P) in the cells.This kit is based on theenzymatic method for the direct measurement of 2DG6P amount without any use of radioisotope materials (Saito K and Minokoshi Y, et al. Analytical Biochem 412: 9-17, 2011).
Storage:
-20°C
Kit Components:
1. Solution A: 1,800 ul x 1 tube;2. Solution B (Acid solution): 500 ul x 1 tube;3. Solution C (Acid neutralizing solution): 500 ul x 1 tube;4. Solution D: 850 ul x 1 tube;5. Solution E (Alkali solution): 500 ul x 1 tube;6. Solution F (Alkali Neutralizing solution): 500 ul x 1 tube;7. Solution G: 1,100 ul x 1 tube;8. 1 mM 2DG6P solution: 250 ul x 1 tube;9. Sample diluent buffer Concentrate (100-fold concentrated solution): 1.5 ml x 1 tube;10. Substrate buffer: 6.5 ml x 1 vial;11. DTNB Substrate (powder): 3 vials;12. Low G6PDH: 13 ul x 1 tube;13. High G6PDH: 130 ul x 1 tube;14. GR: 10 ul x 1 tube


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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