Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Metabolism Kits > ADP Colorimetric/Fluorometric Assay Kit

ADP Colorimetric/Fluorometric Assay Kit

"ADP" Related Products.
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His & S(1)
Product Overview:
Assay for the quantification of cellular ADP
1 kit/100 assays
ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. The newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (λmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 μM ADP in biological samples.
In the assay, ADP is converted to ATP and pyruvate. The assay can detect as low as 1 μM ADP in biological samples.
Target Species:
For research use only. Not intended for any diagnostic or therapeutic purpose.
Store at -20°C. Product has an expected shelf life of 12 months.
Kit Components:
ADP Assay Buffer;ADP Probe (lyophilized);DMSO (anhydrous);ADP Converter;ADP Developer Mix;ADP Standard (1 μmole lyophilized)
Detection method:
Colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm)
Compatible Sample Types:
Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits:
1. Simple procedure; takes less than 1 hour.2. Fast and convenient.3. The assay is sensitive, stable and high-throughput adaptable.

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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