AHCY Inhibitor Screening Kit
Cat.No. : | Kit-2151 |
Product Overview : | In the AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent. |
- Specification
- Gene Information
- Related Products
Size : | 100 assays |
Applications : | Screening/characterizing/studying AHCY inhibitors |
Storage : | Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay.AHCY Assay Buffer: Store at 4°C or -20°C. Warm to 37°C before use.AHCY Enzyme: Reconstitute with 220 µl AHCY Reconstitution Buffer, keep on ice, and mix gently by pipetting. Aliquot and store at -80°C. Avoid repeated freeze/thaw. After opening, use within two months.Adenosine Deaminase: Reconstitute with 110 µl deionized water. Avoid repeated freeze/thaw. Aliquot and store at -80°C. After opening, use within two months.3-Deazaneplanocin A (10 µM): Store at -20°C. After opening, use within two months.AHCY Substrate: Store at -20°C. Thaw and mix gently by pipetting before use. After opening, use within two months.Thiol detecting Reagent: Store at -20°C. Thaw before use. After opening, use within two months. |
Products Types
◆ Recombinant Protein | ||
AHCY-403M | Recombinant Mouse AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
AHCY-519H | Recombinant Human AHCY Protein, MYC/DDK-tagged | +Inquiry |
AHCY-99H | Recombinant Human AHCY Protein, His-tagged | +Inquiry |
AHCY-294H | Recombinant Human AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
AHCY-35C | Recombinant Cynomolgus Monkey AHCY Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
AHCY-8964HCL | Recombinant Human AHCY 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2302 | Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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