Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > AMPK Phosphorylation Assay Kit

AMPK Phosphorylation Assay Kit

   Share
"AMPK" Related Products.
Cat.No.:
Kit-0088
Product Overview:
AMPK Phosphorylation Assay Kit is an indirect enzyme-linked immunoassay for qualitative determination of AMPK phosphorylation in cultured cells. at FL360/450, 530/585 nm.
Description:
The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of α- (63 kDa), β- (38 kDa) and γ- (38 kDa) subunits. For each subunit, isoforms have been identified (α1, α2, β1, β2, γ1, γ2, γ3) which theoretically allow the formation of 12 different proteins. The α- subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP (γ-subunit) and for glycogen (β-subunit). AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the γ-subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphorylation of Thr-172. AMPK Phosphorylation Assay Kit measures phosphorylated AMPK in whole cells and normalizes the signal to the total protein content. The antibody recognizes both α-subunits and thus can be used for cells from all tissues (human, mouse, rat). This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study AMPK regulation in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. AMPK phosphorylation (pAMPK) is measured using a fluorescent ELISA followed by total protein measurement in each well.
Applications:
Determination of AMPK phosphorylation status in whole cells.Evaluation of direct and indirect modulation of AMPK phosphorylation.Species tested: human, mouse, rat.
Target Species:
Human, Mouse, Rat
Usage:
For research use only (RUO)
Storage:
Upon delivery, store all reagents at -20°C. Shelf life: 6 months after receipt.
Kit Components:
10x Wash Buffer 25 mLProtein Stain 6 mLAb1 10 μLBlocking Buffer 25 mLHRP Substrate 6 mLAb2 10 μL
Detection method:
Fluorimetric
Compatible Sample Types:
Cell
Features & Benefits:
Sensitive. Can measure pAMPK modulation in as little as 500 cells/well.Simple and convenient. No cell lysis necessary, cells can be cultured for several days.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.