Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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AOZ chromatographic diagnostic kit

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Cat.No.:
Kit-1896
Product Overview:
The AOZ chromatographic diagnostic kit is based on the specific immuno-chemical reactions between antigen and antibody. It relies on the competition between AOZ residues in the sample and the AOZ immobilized on T line on the membrane for the AOZ antibody-dye conjugate. Since the drug has been presenting in the sample, it will compete with the drug immobilized on T line, to the limited amount of dye-antibody. As a sufficient amount of drug in the sample is presenting, the drug will saturate the antibody. Consequently, it will show an extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a negative sample (or the amount of the drug is lower than the minimum detectable concentration, 0.1 ppb), it will generate two obvious lines in both the T and C line section.
Description:
Furazolidone is one of the nitrofurans which are synthetic broad-spectrum antibiotics, and often used for their antibacterial properties in food-producing animals. Nitrofurans have been banned on treating animals used for food production, because of the possibility of increased cancer risk if people are exposed to them over a long period of time. The European Union banned the use of nitrofurans in animal food production in 1995 with the United States following suit in 2002. Going beyond the ban on the use of nitrofuran, the EU implemented a stricter food import inspection policy after nitrofuran residues were found in poultry, fish and shrimp. The analysis of residues of nitrofuran drugs needs to be based on the detection of the tissue bond metabolites of the nitrofuran parent drugs. Since the parent drugs are very rapidly metabolized, they are not detectable shortly after treatment. The tissue bound nitrofuran metabolites are detectable for a long time after administration and therefore they are used for the detection of the abuse of nitrofurans. Nitrofuran metabolites are found after administration of Furazolidone (metabolite:3-amino-2-oxazolidinone = AOZ), Furaltadone (metabolite:3-amino-5-morpholino-methyl-2-oxazolidinone = AMOZ), Nitrofurantoin (metabolite:1-aminohydantoin = AHD) and Nitrofurazone (metabolite:semicarbazide = SEM).
Kit Components:
1. Test cassette2. Desiccant3. Dropper4. 10X Sample buffer (1 mL/ tube)5. Derivative reagent:1 bt/kit, 8 ML/bt
Compatible Sample Types:
Fish; Shrimps; Chicken; Pork; Honey
Features & Benefits:
• No instrumentation required• High specificity• High sensitivity• Highly reproducibility• Quick results
Sensitivity:
Sample Cut-off (ppb)Fish, Shrimps, Chicken, Pork, Honey 0.1


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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