Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > ATP synthase Specific Activity Microplate Assay Kit

ATP synthase Specific Activity Microplate Assay Kit

"ATP synthase" Related Products.
Product Overview:
Rapid multiplexing microplate kit is used to determine both the activity and quantity of ATP synthase (Complex V) in a Human or Rat sample. The ratio of the two measurements represents the enzyme"s specific activity. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidative reaction of NADH to NAD+. The formation of NAD+ results in a decrease in absorbance at 340 nm. Subsequently, in these same wells, the quantity of ATP synthase is determined by adding an ATP synthase specific antibody conjugated with alkaline phosphatase. This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner proportional to the amount of protein captured in the wells. Included for optimal performance of the assay are buffer, detergent, antibody, substrate, lipid mix and a 96-well microplate pre-coated with monoclonal antibody.
1 x 96 well plate
Functional Studies
Target Species:
Reacts with: Rat, Cow, HumanDoes not react withMouse
Store Buffer, Detergent, Detector Antibody, 2500X AP Label, AP Buffer, and the microplate at 4°C. Store the Reagent Mix, AP Development Reagent and Lipid Mix at -20°C. For multiple experiments, the Reagent Mix and AP Development Reagent, and Lipid Mix may be aliquoted and stored at -80°C to minimize freeze
Kit Components:
Components Identifier 96 testsAP ATP synthase Detector antibody 1 x 1mlAP Buffer Tube 2 1 x 20mlAP Development Reagent 1 x 400µlAP label 2500 X Tube B 1 x 10µlATP synthase precoated Microtiter plate 1 unitBuffer Tube 1 1 x 15mlDetergent 1 x 1mlLipid Mix 1 x 6mlReagent Mix 1 x 20ml
Detection method:
Compatible Sample Types:
Cell culture supernatant, Cell culture extracts, Tissue, Tissue Extracts, Cell Lysate

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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