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ATPase Kinetic Assay Kit

Cat.No. : Kit-0118
Product Overview : There are a multitude of enzymes that hydrolyze ATP or GTP to form ADP or GDP and inorganic phosphate (Pi). The Enzyme Linked Inorganic Phosphate Assay (ELIPA) from Cytoskeleton Inc. allows one to measure the phosphate released during hydrolysis on a real time basis. Thus a kinetic assay technique is produced for your enzyme"s activity. Particular purified enzymes that are applicable to this analysis are signaling phosphatases, apyrase, kinesin motors, metabolic enzymes, membrane transporters and alkaline phosphatase. Generally this assay is useful for enzymes with Kcat above 0.1, if your enzyme or the family of enzymes has a lower Kcat then the CytoPhosTM Phosphate Assay is useful. If your enzyme has a very low activity i.e. small G-proteins, the non-radioactive GAP assay or the radioactive alternative, EasyRad Phosphate Assay is ideal.The assay is an adaptation of a method originally described by Webb for the measurement of glycerol kinase plus D-glyceraldehyde ATPase activity and for actin activated myosin ATPase. The assay is based upon an absorbance shift (330-360 nm) that occurs when 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methyl purine in the presence of inorganic phosphate (Pi). The reaction is catalyzed by purine nucleoside phosphorylase (PNP). One molecule of inorganic phosphate will yield one molecule of 2-amino-6-mercapto-7-methyl purine in an essentially irreversible reaction. Thus, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the reaction.
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Size : 96 assays
Applications : 1. Kinetic measurement of ATPase and GTPase activities.2. Discovery and characterization of ATPase/GTPase inhibitors.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Is ATPase involved in specific cellular functions? 09/04/2020

Yes, ATPase plays a crucial role in various cellular processes such as active transport, muscle contraction, and cell signaling.

How does ATPase contribute to active transport? 08/19/2019

ATPase provides the energy needed for active transport by hydrolyzing ATP to ADP, facilitating the movement of ions or molecules across cell membranes against their concentration gradients.

Is there tissue-specific expression of ATPase isoforms? 02/19/2018

Yes, different tissues may express specific isoforms of ATPase tailored to their energy requirements and cellular functions.

What is the general function of ATPase? 11/06/2017

ATPase is an enzyme that catalyzes the hydrolysis of ATP to ADP, releasing energy for various cellular processes.

Where is ATPase typically found in cells? 10/23/2017

ATPase is often associated with cell membranes, organelles, and cellular structures involved in energy-dependent processes.

Are there different types of ATPases? 10/18/2017

Yes, ATPases are classified into different types based on their subunit composition and cellular functions, including F-type, V-type, and P-type ATPases.

Can ATPase be inhibited for therapeutic purposes? 05/09/2016

In certain medical conditions, inhibiting ATPase activity is explored as a potential therapeutic strategy, particularly in diseases related to ion transport and cellular energetics.

Customer Reviews (3)

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Reviews
04/26/2021

    The product's user-friendliness made it a favorite among team members, enhancing collaboration.

    07/21/2020

      Its ease of use allowed us to focus more on data analysis and interpretation, rather than technical challenges.

      10/29/2018

        It met stringent safety standards, allowing us to work with confidence in a controlled laboratory environment.

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