Acetate Assay Kit is a quantitative colorimetric/fluorimetric determination of acetate at OD570, FL530/585 nm.
ACETATE is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. Acetate Assay Kit uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
Direct Assays: acetate in biological samples such as serum/plasma, in food, agriculture and environmental samples.Drug Discovery/Pharmacology: effects of drugs on acetate metabolism.
For research use only (RUO)
Store all components at -20°C. Shelf life of six months after receipt.
Assay Buffer 25 mLDeveloper 1 mLDye Reagent 120 µLATP 120 µLEnzyme A DriedEnzyme B DriedStandard 1 mL
Compatible Sample Types:
Agriculture, Environment Samp, Food, Plasma, Serum
Features & Benefits:
Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.