Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Epigenetic Kits > Acetyl Histone H4K5 Quantification Kit (Colorimetric)

Acetyl Histone H4K5 Quantification Kit (Colorimetric)

"Acetyl Histone" Related Products.
Product Overview:
Acetyl Histone H4K5 Quantification Kit (Colorimetric) is used for measuring acetylation of histone H4K5.
Acetylation of histones, including histone H3 and H4, has been involved in the regulation of chromatin structure and recruitment of transcription factors to the gene promoters. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) play a critical role in the control of histone H4 acetylation at multiple sites. Acetylation of histone H4 at lysine 5 (H4K5) reflects the hyperacetylated state in histone H4 and is strongly correlated with active states of genes. H4K5 acetylation is considered to be a marker of dexamethasone transactivation and the change of H4K5 acetylation is observed in cancer and inflammatory diseases. Histone H4K5 acetylation may be increased by inhibition of HDACs and decreased by HAT inhibition; thus, quantitative detection of acetyl histone H4K5 would provide useful information for better understanding epigenetic regulation of gene activation, and for developing HAT or HDAC-targeted drugs. Acetyl Histone H4K5 Quantification Kit (Colorimetric) provides a tool for measuring acetylation of histone H4K5.
Acetyl Histone H4K5 Quantification Kit (Colorimetric) is suitable for specifically measuring histone H4K5 acetylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells.
For research use only (RUO)
Upon receipt, store C3 and standard control at –20°C. Store all other components at 4°C away from light. The components of the kit are stable for 6 months when stored properly.Note: Check if buffers C1 and C2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are re-dissolved.
Kit Components:
C1 (10X wash buffer) 10 mlC2 (antibody buffer) 6 mlC3 (detection antibody, 1 mg/ml)* 5 µlC4 (color developer) 5 mlC5 (stop solution) 3 mlSignal report solution* 5 µlSignal enhancer* 120 µlStandard control (100 µg/ml)* 10 µl8 well sample strips (with frame) 48 well standard control strips 2User guide 1* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
Detection method:
Compatible Sample Types:
Histone Extract
Features & Benefits:
Quick and efficient procedure, which can be finished within 2.5 hours.Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.Specifically captures acetyl H4K5 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.The control is conveniently included for the quantification of the amount of acetyl H4K5.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.