Acetyl Histone H4K8 Quantification Kit (Fluorometric) is used for measuring acetylation of histone H4K8.
Acetylation of histones, including histone H3 and H4, has been involved in the regulation of chromatin structure and recruitment of transcription factors to the gene promoters. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) play a critical role in the control of histone H4 acetylation at multiple sites. Acetylation of histone H4 at lysine 8 (H4K8) reflects the hyperacetylated state in histone H4 and is strongly correlated with active states of genes. H4K8 acetylation is related to DNA repair and increased H4K8 acetylation is observed in cancer and inflammatory diseases. In the absence of this modification, cellular survival following DNA damage is impaired. Histone H4K8 acetylation may be increased by inhibition of HDACs and decreased by HAT inhibition; thus, quanti-tative detection of acetyl histone H4K8 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HAT or HDAC-targeted drugs. Acetyl Histone H4K8 Quantification Kit (Fluorometric) provides a tool for measuring acetylation of histone H4K8.
Acetyl Histone H4K8 Quantification Kit (Fluorometric) is suitable for specifically measuring histone H4K8 acetylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells.
For research use only (RUO)
Upon receipt, store F4 and standard control at –20°C. Store all other components at 4°C away from light. The components of the kit are stable for 6 months when stored properly.Note: Check if buffers F1 and F2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are re-dissolved.
F1 (10X wash buffer) 10 mlF2 (antibody buffer) 6 mlF3 (detecting antibody, 1 mg/ml)* 5 µlF4 (fluoro developer)* 12 µlF5 (fluoro enhancer)* 12 µlF6 (fluoro dilution) 4 mlStandard control (100 µg/ml)* 10 µlSignal report solution* 5 µlSignal enhancer* 120 µl8 well sample strips (with frame) 68 well standard control strips 2User guide 1* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
Compatible Sample Types:
Features & Benefits:
Quick and efficient procedure, which can be finished within 2.5 hours.Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.Specifically captures acetyl H4K8 with the detection limit as low as 0.4 ng/well and detection range from 20 ng-5 µg/well of histone extracts.The control is conveniently included for the quantification of the amount of acetyl H4K8.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions.