Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Acetylcholinesterase Activity Colorimetric Assay Kit

Acetylcholinesterase Activity Colorimetric Assay Kit

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GST(2)
His(11)
MYC/DDK(1)
Cat.No.:
Kit-0031
Product Overview:
Rapid, simple & convenient
This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples
Description:
Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.
Applications:
Measurement of AChE activity in various tissues/cells.
Usage:
For Research Use Only! Not For Use in Humans.
Storage:
-20°C
Size:
100 assays
Kit Components:
• AChE Assay Buffer
• AChE Probe (DMSO)
• AChE Substrate (Lyophilized)
• Choline Oxidase Enzyme Mix (Lyophilized)
• Choline Standard (Lyophilized)
• AChE Positive Control (Lyophilized)
Target Species:
N/A
Detection method:
Absorbance (OD 570 nm)
Compatible Sample Types:
• Serum, plasma or blood.
• Animal tissues: liver, heart, kidney, etc.
• Cell culture: Adherent or suspension cells


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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