Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. (500 assays) The assay detects acid phosphatase (AP) activity conveniently in various biological samples.
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The Acid Phosphatase Activity kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
AP Assay BufferpNPP Substrate (10 TAB)AP EnzymeStop Solution
Compatible Sample Types:
Cell Culture SupernatantsPlasmaSerumTissue LysatesUrineCell Lysates