Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Acidic Mammalian Chitinase Inhibitor Screening Kit

Acidic Mammalian Chitinase Inhibitor Screening Kit

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Cat.No.:
Kit-2104
Product Overview:
Acidic Mammalian Chitinase Inhibitor Screening Kit uses Bisdionin F— a highly selective AMCase inhibitor as positive control and it provides a simple, rapid, sensitive and reliable test suitable for screening/characterizing AMCase inhibitors.
Size:
100 assays
Description:
Acidic Mammalian Chitinase (AMCase) belongs to the glycosyl hydrolase-18 family (EC 3.2.1.14), which is capable of hydrolyzing the β- (1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. AMCase is relatively abundant in the gastrointestinal tract and lung. It is highly expressed in the lungs of asthmatic patients and asthma animal models. In addition, the change in AMCase expression can be used to monitor Th-2/IL-13 mediated immune response and inflammation. Inhibition of AMCase activity has been considered as a therapeutic strategy to alleviate symptoms of allergic airway inflammation. Thus, there is a need to identify AMCase inhibitors as potential candidates to treat and/or prevent asthma.
Applications:
Screening/characterizing/studying Mammalian Chitinase inhibitors/activators
Storage:
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing theassay.AMCase Assay Buffer: Warm to 37 ºC before use. Store at either 4ºC or -20ºC.AMCase Substrate: Store at -20ºC. Bring to room temperature before use.Acidic Mammalian Chitinase: Reconstitute Acidic Mammalian Chitinase in 550 μl AMCase Assay Buffer and mix thoroughly. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use. Use within two months.Bisdionin F: Store at -20ºC. Bring to room temperature before use.
Kit Components:
AMCase Assay Buffer: 25 mlAMCase Substrate (in DMSO): 25 µlAcidic Mammalian Chitinase (lyophilized): 1 vialBisdionin F (5 mM): 20 µl
Detection method:
Fluorescence (RFU= 320/445 nm)
Features & Benefits:
• Simple and reliable test to screen AMCase inhibitors/activators• High-throughput compatible• Includes Inhibitor Control (Bisdionin F)


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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