Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
Aconitase (aconitate hydratase; EC 18.104.22.168) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.
Store kit at -20ºC (except the Enzyme Mix which should be stored at 4ºC) in the dark immediately upon receipt. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.
Components Identifier 100 tests(NH4)2Fe (SO4)2 (Lyophilised) Brown/NM 1 vialAssay Buffer WM 1 x 30mlCysteine (Lyophilised) Red 1 vialDeveloper (Lyophilised) Purple 1 vialEnzyme Mix Green 1 x 200µlIsocitrate Standard (100 mM) Yellow 1 vialSubstrate (lyophilized) Blue 1 vial
Compatible Sample Types:
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts