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Adenosine Deaminase Fluorometric Activity Assay Kit

Cat.No. : Kit-2148
Product Overview : ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.
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Size : 100 assays
Applications : Detection of Adenosine Deaminase activity
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.ADA Assay Buffer (10x): Make 1x Assay Buffer by adding one part 10x Assay Buffer to nine parts deionized water. Store at -20°C or 4°C. Bring to 37°C before use.ADA Convertor and ADA Developer: Reconstitute each with 210 µl ADA Assay Buffer and mix gently by pipetting. Briefly centrifuge to collect the contents at the bottom of the tube. Aliquot and store at -20°C. Avoid repeated freeze/thaw.ADA Substrate: Aliquot and store at -20°C. Avoid repeated freeze/thaw.ADA Positive Control: Reconstitute with 22 µl ADA Assay Buffer and mix gently by pipetting. Briefly centrifuge to collect the contents at the bottom of the tube. Aliquot and store at -20°C. Avoid repeated freeze/thaw.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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