Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Akt1/PKBα Immunoprecipitation-Kinase Assay Kit

Akt1/PKBα Immunoprecipitation-Kinase Assay Kit

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Cat.No.:
Kit-0067
Product Overview:
Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB.
Size:
1 kit
Description:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Applications:
Application: Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB.Key Applications: Kinase Assay; Immunoprecipitation.
Kit Components:
P81 Phosphocellulose Squares;Assay Dilution Buffer, 5X;Anti-Akt/PKB, PH Domain, clone SKB1;PKA Inhibitor peptide;Magnesium/ATP Cocktail
Detection method:
Radioactive


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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