Detection and Quantification of Alanine Transaminase Activity.
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (sGPT), facilitates the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage.
Shipped and store at 4°C for 6 months.
Component-Volume-Storage 1. 96-Well Microplate;2. Extract: 30 ml x 1, 4°C; 3. Reagent I: 5 ml x 1, 4°C; 4. Reagent II: 7.5 ml x 1, 4°C; 5. Reagent III: 25 ml x 1, 4°C; 6. Technical Manual: 1 Manual
Compatible Sample Types:
Cell culture supernatant; Urine; Serum; Plasma; Other biological fluids; Tissue Extracts; Cell culture media