Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Alcohol Dehydrogenase Detection Kit

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Cat.No.:
Kit-0052
Product Overview:
Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Description:
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
Applications:
Functional Studies
Storage:
Store the kit at -20°C, protect from light. Allow ADH Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.DEVELOPER: Reconstitute Developer with 0.9 ml of ddH2O. Pipette up and down several times to completely dissolve the pellet into solution. (Do not vortex).
Kit Components:
Components Identifier 100 testsADH Assay Buffer WM 1 x 25mlADH Positive Control (Lyophilized) Green 1 vialDeveloper (Lyophilized) Red 1 vialIsopropanol Blue 1 x 1mlNADH Standard (Lyophilized) Yellow 1 vial
Compatible Sample Types:
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
Sensitivity:
0.01 mU/well


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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