"ADH" Related Products

Alcohol Dehydrogenase Detection Kit

Cat.No.: Kit-0052
Product Overview: Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Description: Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
Applications: Functional Studies
Storage: Store the kit at -20°C, protect from light. Allow ADH Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.DEVELOPER: Reconstitute Developer with 0.9 ml of ddH2O. Pipette up and down several times to completely dissolve the pellet into solution. (Do not vortex).
Kit Components: Components Identifier 100 testsADH Assay Buffer WM 1 x 25mlADH Positive Control (Lyophilized) Green 1 vialDeveloper (Lyophilized) Red 1 vialIsopropanol Blue 1 x 1mlNADH Standard (Lyophilized) Yellow 1 vial
Compatible Sample Types: Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media
Sensitivity: 0.01 mU/well

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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