Alcohol Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 188.8.131.52) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
Store the kit at -20°C, protect from light. Allow ADH Assay Buffer to warm to room temperature before use. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.DEVELOPER: Reconstitute Developer with 0.9 ml of ddH2O. Pipette up and down several times to completely dissolve the pellet into solution. (Do not vortex).
Components Identifier 100 testsADH Assay Buffer WM 1 x 25mlADH Positive Control (Lyophilized) Green 1 vialDeveloper (Lyophilized) Red 1 vialIsopropanol Blue 1 x 1mlNADH Standard (Lyophilized) Yellow 1 vial
Compatible Sample Types:
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media