Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Alkaline Phosphatase Assay Kit

Alkaline Phosphatase Assay Kit

   Share
"ALP" Related Products.
By Species
Calf(1)
Pig(1)
By Source
Cat.No.:
Kit-0079
Product Overview:
Alkaline Phosphatase Assay Kit is a quantitative fluorimetric determination of alkaline phosphatase at FL360/450 nm.
Description:
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This improved method utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into a highly fluorescent product 4-methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.
Applications:
Direct Assays: ALP activity in serum, plasma and other sources.Characterization and Quality Control for ALP production.Drug Discovery: high-throughput screen for ALP modulators.
Usage:
For research use only (RUO)
Storage:
Store at -20°C. Shelf life of 2 years after receipt.
Kit Components:
Reagent(pH 10.5) 14 mL100x Standard 120 µL
Detection method:
Fluorimetric
Compatible Sample Types:
Plasma, Serum
Features & Benefits:
High sensitivity and wide linear range. Use 10 µL sample. Detection limit of 0.02 U/L (20 min reaction).Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes.Robust and amenable to HTS. All reagents are compatible with high- throughput liquid handling instruments.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.