Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > Angiotensin I Converting Enzyme Inhibitor Screening Kit

Angiotensin I Converting Enzyme Inhibitor Screening Kit

   Share
"ACE1" Related Products.
Cat.No.:
Kit-2113
Product Overview:
ACE1 Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE1 activity to regulate hypertension. It utilizes the ability of an active ACE1 to cleave a synthetic oaminobenzoyl peptide (Abz-based peptide substrate to release a free fluorophore). The released Abz can be easily quantified using a fluorescence microplate reader. In the presence of an ACE1 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE1 inhibitors in a highthroughput format.
Size:
100 assays
Description:
Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. In addition, elevated levels of ACE1 are found in sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher disease, pneumoconiosis, histoplasmosis and miliary tuberculosis.
Applications:
Screening/studying/characterizing inhibitors of ACE1
Storage:
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.• ACE1 Assay Buffer and ACE1 Dilution Buffer: Store at -20 °C or 4 °C. Bring to room temperature before use.• ACE1 Enzyme: Store at -20°C. Avoid multiple freeze/thaw of the enzyme. Use within 6 months.• ACE1 Substrate: Ready to use. Store at -20°C. Thaw before use.• ACE1 Inhibitor: Ready to use. Store at -20°C. Thaw before use.
Kit Components:
ACE1 Assay Buffer: 20 mlACE1 Dilution Buffer: 1 mlACE1 Enzyme: 50 µlACE1 Substrate: 300 µlACE1 Inhibitor (10 mM Captopril): 100 µl
Detection method:
Fluorometric (Ex/Em = 330/430 nm)
Compatible Sample Types:
Small Molecules/Ligands
Features & Benefits:
• Simple, rapid assay.• Takes only 1-2 hrs• Non-radiometric fluorescent screening process• HTP adaptable


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.