Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Anoikis Assay kit

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Cat.No.:
Kit-2093
Product Overview:
The Anoikis assay kit quantifies live cells using fluorometric (Calcein AM) reagents and a proprietary coated chamber that mimics Anoikis conditions. Our assay is easy to use, sensitive and compatible with fluorimeters.
Size:
100 assays
Description:
Anoikis is a form of cellular apoptosis induced by the inability of normal cells to attach to the extracellular matrix (ECM). Incorrect attachment or no ECM attachment could affect cell growth and differentiation in normal cells. However, research studies has determined that metastatic cells can undergo Anoikis without affecting their ability to migrate and invade surrounding or distant organs.
Applications:
Measure cell anoikis in response to stimuli.Screen, study, or characterize compounds that influence Anoikis.HTP adaptable
Target Species:
Mammalian cells
Storage:
Store kit at -20°C, protected from light. Components should be opened and kept under sterile conditions. Briefly centrifuge small vials prior to opening. Assay is performed under sterile conditions. Read entire protocol before performing the experiment.Anoikis Chamber: Ready to use. Keep at Room temperature.Calcein AM: Resuspend in 220 µl anhydrous DMSO (not provided). Aliquot and store -20°C. Use within 2 months.Assay Buffer: Ready to use. Keep at 4°C.
Kit Components:
Assay Buffer: 100 mlAnoikis Chamber: 1 eachChamber Control (Uncoated): 1 eachCalcein AM Dye: 1 vial
Detection method:
Fluorescence (Ex/Em = 485/530 nm)
Compatible Sample Types:
• Metastatic cell lines• Anoikis inhibitor or stimuli
Features & Benefits:
• Highly sensitive fluorometric method to measure cell Anoikis in response to a variety of biochemical stimuli• Simple & High throughput-adaptable• Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Anoikis


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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