Ascorbic Acid Assay Kit is a quantitative colorimetric/fluorometric ascorbic acid determination.
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
Assays: ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.Drug Discovery/Pharmacology: effects of drugs on ascorbic acid metabolism.
For research use only (RUO)
Store all components at -20°C. Shelf life of 12 months after receipt.
Assay Buffer 10 mLEnzyme Mix 120 μLDye Reagent 120 μLStandard 400 μL 10 mM ascorbic acid
Compatible Sample Types:
Cell Culture, Milk, Plasma, Saliva, Serum, Tissue, Urine
Features & Benefits:
Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.