Asparaginase Activity Assay Kit is a colorimetric and fluorometric method for assaying the activity of asparaginase.
Asparaginase (EC 18.104.22.168) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme"s antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. Asparaginase Activity Assay Kit provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
For research use only (RUO)
Store kit at -20°C.
Asparaginase Assay Buffer 25 mlOxiRed Probe (in DMSO) 0.2 mlSubstrate Mix (lyophilized) 1 vialAspartate Enzyme Mix (lyophilized) 1 vialConversion Mix (lyophilized) 1 vialPositive Control (lyophilized) 1 vialAspartate Standard (100 mM) 0.1 ml
Compatible Sample Types:
Cell Lysate, Biological Sample, Food Sample