Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > Autotaxin Inhibitor Screening Assay Kit

Autotaxin Inhibitor Screening Assay Kit

"Autotaxin" Related Products.
96 wells
Autotaxin (ATX, Ectonucleotide Pyrophosphatase/Phosphodiesterase-2, ENPP-2, Lysophospholipase D) is a secreted lysophospholipase D that catalyzes the hydrolysis of lysophosphatidylcholine (LPC) to generate lysophosphatidic acid (LPA). LPA is a lipid mediator that activates G protein-coupled receptors and induces a variety of biological responses, such as neurogenesis, angiogenesis, smooth-muscle contraction, platelet aggregation, and wound healing. ATX-LPA signaling is involved in a range of pathologies including tumor progression and inflammation. The Autotaxin Inhibitor Screening Assay provides a convenient method for screening human ATX inhibitors. ATX cleaves bis-(p-nitrophenyl) phosphate liberating p-nitrophenol, a yellow product that is measured at 405-415 nm.
Kit Components:
Autotaxin Assay Buffer (10X): 1 vial/5 ml; -20°CAutotaxin (human recombinant) Assay Reagent: 2 vials/60 µl; -80°CAutotaxin Substrate: 2 vials/lyophilized; -20°C96-Well Solid Plate (Colorimetric Assay): 1 plate; Room temperature96-Well Cover Sheet: 1 cover; Room temperature

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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