"BCL-XL" Related Products

BCL-XL TR-FRET Assay Kit

Cat.No.: Kit-1687
Size: 384 reactions
Description: The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Storage: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: BCL-XL: 20 µg; -80°CBCL-XL Peptide Ligand: 50 µl; -80°CAnti-His Tb donor: 2 x 10 µl; -20°CDye-labeled acceptor: 2 x 10 µl; -20°C3x BCL TR-FRET Assay Buffer: 4 ml; -20°CWhite, nonbinding, low volume, microtiter plate: 1; Room temp.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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