The BCL-XL TR-FRET Assay Kit is designed to measure the inhibition of BCLXL binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL-XL, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
BCL-XL: 20 µg; -80°CBCL-XL Peptide Ligand: 50 µl; -80°CAnti-His Tb donor: 2 x 10 µl; -20°CDye-labeled acceptor: 2 x 10 µl; -20°C3x BCL TR-FRET Assay Buffer: 4 ml; -20°CWhite, nonbinding, low volume, microtiter plate: 1; Room temp.