BCP Albumin Assay Kit is a quantitative colorimetric albumin determination at 610 nm.
Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium, magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration.
Direct Assays: albumin in serum, urine, biological preparations.Drug Discovery/Pharmacology: effects of drugs on albumin metabolism.
For research use only (RUO)
Store Reagent and standard at 4°C and -20°C, respectively. See expiry dates on labels.
Reagent 50 mLAlbumin standard 2 mL 5 g/dL BSA
Compatible Sample Types:
biological preparations, serum, urine
Features & Benefits:
Sensitive and accurate: Use as little as 20 μL samples. Detection range 0.3 g/dL (45 μM) to 5 g/dL (750 μM) albumin in 96-well plate assay.Simple and high-throughput: The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility: The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.