The BPTF TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.
BPTF(FALZ), GST-tag: 10 µg; -80°CBET Bromodomain Ligand: 50 µl; -80°C Non-acetylated Ligand 1: 15 µl; -20°C Tb-labeleddonor: 2x10 µl; -20°C Dye-labeled acceptor: 2x10 µl; -20°C 3x ATAD2A Assay Buffer: 4 ml; -20°C White Nonbinding low volume microtiter plate: 1; Room temp.