The BRD1 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD1 binding to its substrate. The key to the BRD1 Inhibitor Screening Assay Kit is the specific binding of the BRD1 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
BRD1, GST-tag: 24 µg; -80°CBET Ligand 1: 400 µl; -80°CNon-acetylated BET Ligand 1: 200 µl; -80°C3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C