Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Epigenetic Kits > BRD3 bromodomain 1 TR-FRET Assay Kit

BRD3 bromodomain 1 TR-FRET Assay Kit

"BRD3" Related Products.
By Species
Rhesus Macaque(1)
By Source
By Tag
9600 wells
Bromodomains recognize acetylated lysine residues and recruit regulatory complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. The isolated individual or tandem bromodomains of many BET family members, including BRD2, BRD3, BRD4, and BRDT, have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of bromodomain interactions hold promise as useful therapeutics for human disease. The BRD3 bromodomain 1 TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay kit is robust, displaying a Z" >0.6.
Kit Components:
BRD3 bromodomains 1 Europium Chelate: 1 vial/420 wells; 5 vials/420 wells; 5 vials/2,100 wells; -80°CBRD3 bromodomains 1 Ligand/APC Acceptor Mixture: 1 vial/420 wells; 5 vials/420 wells;5 vials/2,100 wells; -80°CTR-FRET Assay Buffer (10X): 1 vial/2 ml; 1 vial/10 ml; 5 vials/10 ml; -20°CTR-FRET Assay Buffer Additive: 1 vial/200 mg; 1 vial/1 g; 5 vials/1 g; -20°C(+)-JQ1 Positive Control*: 1 vial/8 nmol; 5 vials/8 nmol; 5 vials/40 nmol; -80°C384-Well Solid Plate (low volume; black): 1 plate; 5 plates; 25 plates; Room temperatureFoil Plate Covers: 1 cover; 5 covers; 25 covers; Room temperature

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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