The BRD9 Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD9 binding to its substrate. The key to the BRD9 Inhibitor Screening Assay Kit is the specific binding of the BRD9 bromodomain to an acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD9 and an inhibitor of choice is incubated with the biotinylated substrate. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
GST-BRD9: 5 µg; -80°CBET Bromodomain Ligand: 400 µl; -80°CNon-acetylated Ligand 1: 200 µl; -80°C3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C