Beta-Galactocidase Inhibitor Screening Kit, β-Gal converts β-Gal substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase. The assay is simple, high-throughput adaptable and can be performed within 30 min.
Introduction: Beta Galactosidase (β-Gal, EC: 220.127.116.11) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio"s Syndrome, a severe birth defect. β-Gal can also be used as a tool to study protein-protein interaction.
Screening/characterizing/studying potential inhibitors of Beta-Galactosidase
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.β-Gal Assay Buffer: Bring to room temperature before use. Store at -20°C or 4°C.β-Gal Substrate: Thaw at room temperature. Aliquot and store at -20°C.β-Galactosidase: Reconstitute with 550 µl β-Gal Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.β-Gal Inhibitor Control: Reconstitute with 200 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.
β-Gal Assay Buffer: 25 mlβ-Gal Substrate (in DMSO): 200 µlβ-Galactosidase: 1 vialβ-Gal Inhibitor Control: 1 vial
Fluorescence (Ex/Em = 480/520 nm)
Compatible Sample Types:
Variable: Inhibitors, small molecules, proteins, etc.
Features & Benefits:
• Simple and High throughput adaptable• 30 minutes short protocol• Detect using a fluorometer or fluorescence microplate reader.