Beta-Galactosidase Staining Kit is used for testing the efficiency of expression vector mediated gene transfer and the regulation of promoters of genes.
The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme β-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ. The β-Galactosidase staining kit utilizes X-gal as the substrate.
For research use only (RUO)
Store kit at 4°C or -20°C, protected from light. Store reconstituted X-gal in -20°C. All components supplied are stable for 1 year.
Fixative Solution (1X). Cap code: NM. 125 mlX-Gal (150 mg, lyophilized). Cap code: green. 1 vialStaining Solution (1X). Cap code: WM. 125 mlStaining Supplement (100X). Cap code: red. 1.5 ml