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Beta-Secretase Activity Assay Kit II

Cat.No.: Kit-0863
Product Overview: Simple procedure; takes ~ 1 hour. Fast and convenient The assay is sensitive, stable and high-throughput adaptable.
Size: 100 assays
Description: β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer"s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.
Applications: Convenient fluorescence method for detecting β-secretase activity in biological and purified samples
Target Species: Mammalian
Usage: For Research Use Only! Not For Use in Humans.
Storage: -20°C
Kit Components: • β-Secretase Extraction Buffer • β-Secretase Reaction Buffer (2X) • β-Secretase Substrate (in DMSO) • Active β-Secretase (Lyophilized) • β-Secretase Inhibitor (in DMSO)
Detection method: Fluorescence (Ex/Em 335-355/495-510 nm)
Compatible Sample Types: Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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