Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Bilirubin Assay Kit

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Pig(1)
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Cat.No.:
Kit-0128
Product Overview:
Bilirubin Assay Kit is a quantitative colorimetric bilirubin determination at 530 nm.
Description:
BILIRUBIN is one of the degradation products of hemoglobin formed when red blood cells die. Bilirubin exists in the insoluble unconjugated form (also indirect bilirubin), or soluble glucuronide conjugated form bilirubin (also direct bilirubin). Conjugated bilirubin moves into the bile canaliculi of the liver and then to the gall bladder. When stimulated by eating, bile (including the conjugated bilirubin) is excreted into the small intestine, where bilirubin is converted into urobilinogen. Bilirubin is a key diagnostic indicator. High levels of bilirubin result when too much hemoglobin is broken down or the removal of bilirubin does not function properly. The accumulation of bilirubin in the body causes jaundice.
Applications:
Direct Assays: total and direct bilirubin in serumPharmacology: effects of drugs on bilirubin metabolism.
Usage:
For research use only (RUO)
Storage:
Store all reagents at 4°C. Shelf life: 12 months after receipt.
Kit Components:
Reagent A 30 mLReagent B 10 mLReagent C 30 mLSaline 50 mLCalibrator (equivalent to 5 mg/dL Bilirubin) 2 mL
Detection method:
Colorimetric
Features & Benefits:
Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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