"COX" Related Products

COX Colorimetric Inhibitor Screening Assay Kit

Cat.No.: Kit-1941
Size: 192 wells
Description: The COX ​Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX ​Colorimetric Inhibitor Screening assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. The COX ​Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.
Storage: -80°C
Kit Components: Assay Buffer (10X): 2 vials; -20°CHeme: 2 vials; -20°CCOX Colorimetric Assay COX-1 (ovine): 1 vial; -80°CCOX Colorimetric Assay COX-2 (human): 1 vial; -80°CArachidonic Acid (substrate): 2 vials; -20°CPotassium Hydroxide: 2 vials; -20°CColorimetric Substrate: 2 vials; -20°C96-Well Plate (Colorimetric Assay): 2 plates; RT96-Well Cover Sheet: 2 covers; RT

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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