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Home > Products > Assay Kits > Activity Kits > COX Colorimetric Inhibitor Screening Assay Kit

COX Colorimetric Inhibitor Screening Assay Kit

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Cat.No.:
Kit-1941
Size:
192 wells
Description:
The COX ​Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX ​Colorimetric Inhibitor Screening assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. The COX ​Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.
Storage:
-80°C
Kit Components:
Assay Buffer (10X): 2 vials; -20°CHeme: 2 vials; -20°CCOX Colorimetric Assay COX-1 (ovine): 1 vial; -80°CCOX Colorimetric Assay COX-2 (human): 1 vial; -80°CArachidonic Acid (substrate): 2 vials; -20°CPotassium Hydroxide: 2 vials; -20°CColorimetric Substrate: 2 vials; -20°C96-Well Plate (Colorimetric Assay): 2 plates; RT96-Well Cover Sheet: 2 covers; RT


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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