The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX Colorimetric Inhibitor Screening assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. The COX Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.
Assay Buffer (10X): 2 vials; -20°CHeme: 2 vials; -20°CCOX-1 (ovine): 1 vial; -80°CCOX-2 (ovine): 1 vial; -80°CArachidonic Acid (substrate): 2 vials; -20°CPotassium Hydroxide: 2 vials; -20°CColorimetric Substrate: 2 vials; -20°C96-Well Plate (Colorimetric Assay): 2; plates RT96-Well Cover Sheet: 2; covers RT