Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. This kit is much more sensitive than the fluorescence-based calcium assays. This kit provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring the intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues. This kit is more sensitive than the fluorescent calcium assays.
This product should be stored desiccated.
Functional Studies more details
Components: 10 x 96 tests; 100% ETOH: 1 x 500µl; Assay Buffer: 1 x 100ml; Coelenterazine Analog: 1 vial
Compatible Sample Types:
Adherent cells, Suspension cells