Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Metabolism Kits > Calcium Quantification Assay kit (Fluorometric)

Calcium Quantification Assay kit (Fluorometric)

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Cat.No.:
Kit-0141
Product Overview:
The kit provides a simple method for detecting calcium in physiology solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).
Size:
200 tests
Description:
This product is intended to be used in vitro with solution-based samples such as cell extracts. If you would like to detect calcium in vivo using live cells, please check our Fluo-8, Rhod-4 or Fura-2 based assays.
Applications:
Functional Studies more details
Storage:
Store at -20°C.
Kit Components:
Components: 200 tests; 300 mM Calcium Standard: 1 x 0.5ml; Assay Buffer: 1 x 10ml; Rhod Red Indicator: 2 vials
Compatible Sample Types:
Cell culture extracts
Assay time:
0h 30m
Sensitivity:
0.03 mM


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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