"Caspase" Related Products

Caspase-12 Fluorometric Assay Kit

Cat.No.: Kit-0158
Product Overview: Fluorometric assay kit for caspase-12 activity
Size: 1 kit/100 assays
Description: Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Target Species: Mammal
Usage: For research use only. Not intended for any diagnostic or therapeutic purpose.
Storage: Store kit at -20°C (Store Cell Lysis Buffer & 2X Reaction Buffer at 4°C after opening). All reagents are Stable for 6 months from date of receipt under proper storage conditions.
Kit Components: Cell Lysis Buffer: 100ml;2X Reaction Buffer: 4*2ml; ATAD-AFC (1mM): 0.5ml; DTT (1M): 0.4ml
Detection method: Fluorecence microtiter plate reader (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
Compatible Sample Types: Cell and tissue lysates
Features & Benefits: 1. Simple one-step procedure; takes only 1-2 hours.2. Fast and convenient.3. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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