Fluorometric assay kit for caspase-4 activity
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
For research use only. Not intended for any diagnostic or therapeutic purpose.
Store at -20°C. Product has an expected shelf life of 6-12 months.
Cell Lysis Buffer;2X Reaction Buffer;LEVD-AFC (1 mM);DTT (1 M)
Fluorecence microtiter plate reader (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
Compatible Sample Types:
Cell and tissue lysates
Features & Benefits:
1. Simple one-step procedure; takes only 1-2 hours.2. Fast and convenient.3. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.