Simple one-step procedure; takes only 1-2 hours Fast and convenient Cathepsin-D assay is straightforward, and can be adapted to 96-well plate assays.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK (Dnp)-D-R-NH2) labeled with MCA. Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.
Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
For Research Use Only! Not For Use in Humans
• CD Cell Lysis Buffer • CD Reaction Buffer • CD Substrate (1mM)
Fluorescence (Ex/Em 328/460)
Compatible Sample Types:
Cell and tissue lysates