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Home > Products > Assay Kits > Activity Kits > Cathepsin E Fluorometric Activity Assay Kit

Cathepsin E Fluorometric Activity Assay Kit

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Product Overview:
Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.
100 assays
Cathepsin E (CTSE, EC: is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers.
Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E
Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.CTSE Assay Buffer and Lysis Buffer: Bring to room temperature before use. Store at -20°C.Human Cathepsin E: Reconstitute with 20 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months.
Kit Components:
CTSE Assay Buffer: 25 mlCTSE Lysis Buffer: 25 mlCTSE Substrate: 0.2 mlHuman Cathepsin E (Positive Control): 1 vialMCA Standard (1 mM): 15 μl
Detection method:
Fluorescence (Ex/Em = 320/420 nm)
Compatible Sample Types:
• Cell and tissue lysates• Purified Cathepsin E
Features & Benefits:
• Rapid & convenient• Detects Cathepsin E activity as low as 1 mU in biological samples

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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