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Cathepsin E Inhibitor Screening Kit

Cat.No.: Kit-2154
Product Overview: Cathepsin E Inhibitor Screening Kit utilizes the ability of Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin E-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the MCA fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin E.
Size: 100 assays
Description: Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of the gastric cancers.
Applications: Screen/study/characterize potential inhibitors of Cathepsin E
Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the experiment.CTSE Assay Buffer: Bring to room temperature before use. Store at 4°C or -20°C.Human Cathepsin E: Reconstitute in 220 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months.
Kit Components: CTSE Assay Buffer: 25 mlHuman Cathepsin E: 1 vialCTSE Substrate: 0.2 mlCTSE Inhibitor (1 mM Pepstatin A in DMSO): 20 μl
Detection method: Fluorescence (Ex/Em = 320/420 nm)
Features & Benefits: • Convenient & Rapid• High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin E• Cathepsin E inhibitor is included in the kit

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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