"Cathepsin" Related Products

Cathepsin G Activity Colorimetric Assay Kit

Cat.No.: Kit-0183
Product Overview: Simple one-step procedure; takes only 1 hour
Fast, robust and convenient
Cathepsin-G assay is straightforward, and can be adapted to 96-well plate assays.
Description: Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In Cathepasin G Activity assay Kit, Cathepsin G will cleave the substrate and release the dye group, pNA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable for the activity of Cathepsin G.
Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin G proteases that can initiate or propagate proapoptotic signals.
Usage: For Research Use Only! Not For Use in Humans
Storage: -20°C
Size: 100 assays
Kit Components: • Assay Buffer
• Substrate
• Cathepsin G Inhibitor
• pNA Standard (0.1 M)
• Positive Control
Target Species: Mammalian
Detection method: Absorbance (405 nm)
Compatible Sample Types: Cell and tissue lysates, serum

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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