"Cell" Related Products

Cell Cytotoxicity Fluorometric Assay Kit

Cat.No.: Kit-0196
Product Overview: Cell Cytotoxicity Assay Kit (Fluorometric) is a non-radioactive and no-wash method assay suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
Description: The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability. Our Cell Cytotoxicity Assay Kit (Fluorometric) provides a fast, simple, accurate and homogeneous assay for the colorimetric or fluorimetric detection of viable cells. This assay is based on the observation that oxidized non-fluorescent blue resazurin is reduced to a red fluorescent dye (resorufin) by accepting an electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.
Applications: This Cell Cytotoxicity Assay Kit (Fluorometric) is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT.
Storage: Keep in freezer and protect from light.
Kit Components: Component A: Assay Solution 20 mL
Compatible Sample Types: Cells
Features & Benefits: Non-Radioactive: No special requirements for waste treatment.Continuous: Easily adapted to automation without mixing or separation.Convenient: Formulated to have minimal hands-on time.Wide Applications: Cell proliferation and cytotoxicity.Sensitive And Accurate: As low as 100 cells can be accurately quantified.Enhanced Value: Less expensive than the sum of individual components.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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