"Cell" Related Products

Cell Fractionation Kit

Cat.No.: Kit-0197
Product Overview: Cell Fractionation Kit is used for reproducible extraction of four subcellular protein fractions (cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample.
Description: This Cell Fractionation Kit is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Target Species: Mammals
Usage: For research use only (RUO)
Storage: Store the kit at -20°C.
Kit Components: Cytosol Extraction Buffer (CEB). Color code: WM. 20 mlMembrane Extraction Buffer-A (MEB-A). Color code: WM. 20 mlMembrane Extraction Buffer-B (MEB-B). Color code: green. 1.2 mlNuclear Extraction Buffer (NEB). Color code: NM. 10 mlDTT (1 M). Color code: blue. 150 µlProtease Inhibitor Cocktail. Add 150 µl of DMSO, and mix well before use. Color code: red. 1 vial
Compatible Sample Types: Cell Culture, Tissue

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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