Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Cellular Kits > Cell Viability Green Fluorescence Assay Kit

Cell Viability Green Fluorescence Assay Kit

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His(1)
Cat.No.:
Kit-0200
Product Overview:
Cell Viability Assay Kit (Green Fluorescence) is a fluorescent assay used to label cells for fluorescence microplate and microscopic investigations of cellular functions.
Description:
There are a variety of parameters that can be used to monitor cell viability. The proprietary green fluorescent dye used in the kit is a hydrophobic compound. It easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The green fluorophore generated by the non-fluorescent substrate used in the kit has the spectral properties of fluorescein at Ex/Em = ~490 nm/520 nm. When well excited with the Argon Laser at 488 nm, the fluoreophore emits intense green fluorescence at ~520 nm.
Applications:
The kit provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays.
Usage:
For research use only (RUO)
Storage:
Store the kit at -20°C and avoid from light.
Kit Components:
Component A: CytoCalcein Green (lyophilized) 5 vialsComponent B: DMSO 200 μLComponent C: Assay Buffer 50 mL
Detection method:
Fluorometric
Compatible Sample Types:
Cells
Features & Benefits:
Robust: Higher maximum signal with lower variation across the plate.Convenient: Formulated to have minimal hands-on time.Rapid Dye Loading: Dye loading at RT for 30 min to 1 hr.Versatile Applications: Compatible with many cell lines and targets.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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